Question

Select the two correct statements out of the four (i -iv) statements given below about lac operon.
(i) Glucose or galactose may bind with the repressor and inactivate it
(ii) In the absence of lactose the repressor binds with the operator region
(iii) The z-gene codes for permease
(iv) This was elucidated by Francois Jacob and Jacques Monod. The correct statements are

• A. (ii) and (iii)
• B. (i) and (iii)
• C. (ii) and (iv)
• D. (i) and (ii).

Answer 1

Answer: (C)

The two French scientists, Jacob and Monod proposed the lac operon of E. coli. The lac operon (an inducible operon) contains a promoter, an operator, a regulator gene and three structural genes z, y, and a, coding for the enzyme β-galactosidase,β-galactoside permease, and β-galactoside transacetylase, respectively. P-galactoside permease 'pumps' lactose into the cell, where P-galactosidase cleaves it into glucose and galactose. The function of the transacetylase is still not clear. The lac regulator gene, designated the i gene, codes for a repressor. In the absence of the inducer (i.e., lactose, actually allolactose), the repressor binds to the lac operator sequence, preventing RNA polymerase from binding to the promoter and transcribing the structural genes. The inducer of the operon, allolactose, is derived from lactose in a reaction that is catalyzed by (β- galactosidase. Once formed, allolactose binds to the repressor, causing it to be released from the operator is doing so, it induces transcription of the z, y and a structural genes. CAP is activator called catabolic activator protein. It exerts a positive control in lac operon because in its absence RNA polymerase is unable to recognise promotor gene. CAP activates lac genes only when glucose is absent. Such enzymes whose synthesis can be induced by adding the substrate are known as inducible enzymes and the genetic systems responsile for the synthesis of such an enzyme are known as inducible operons.