Question

Select the incorrect statement.

• A. More than 900 restriction enzymes have been isolated from over 230 strains of bacteria.
• B. In the year 1963, the two enzymes responsible for restricting the growth of bacteriophage in Escherichia coli were isolated.
• C. Some key tools for recombinant DNA technology are restriction enzyme, polymerase enzyme, ligase, vectors and host root organisms.
• D. EcoRI cut the DNA between bases A and T only when the sequence GAATTC is present in the DNA.

Answer 1

Answer: (D)

EcoRI identifies specific palindromic DNA sequence GAATTC and cuts are specific location away from center between G and A making staggered or sticky end cuts. It breaks phosphodiester bonds and hydrogen bonds.