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Q. Select the correct option regarding restriction endonuclease
P. 900 restriction enzymes have been isolated from over 200 strains.
Q. The first restriction exonuclease was Hind II.
R. Each restriction endonuclease recognises same palindromic sequence.

NTA AbhyasNTA Abhyas 2020Biotechnology : Principles and Processes

Solution:

The first restriction endonuclease-Hind II, whose functioning depended on a specific DNA nucleotide sequence was isolated and characterised five years later. It was found that Hind II always cut DNA molecules at a particular point by recognising a specific sequence of six base pairs. This specific base sequence is known as the recognition sequence for Hind II. Besides Hind II, today we know more than 900 restriction enzymes that have been isolated from over 230 strains of bacteria each of which recognise different recognition sequences. Each restriction endonuclease recognises specific palindromic sequence.