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Q. Radioisotopes used to detect newly synthesized protein is

VMMC MedicalVMMC Medical 2010

Solution:

Proteins are large-sized molecules having one or more polypeptides. Newly synthesized protein is detected by using radioisotope of sulphur $ ({{S}^{35}}) $ . Radioisotope of carbon $ ({{C}^{14}}) $ used to prove the $ C{{O}_{2}} $ reduction in dark reaction of photosynthesis. Heavy isotope of oxygen $ ({{O}^{18}}) $ is used to determine that oxygen in photosynthesis released as a product of photolysis of water. Meselson and Stahl (1958) varified the semi-conservative nature of DNA-replication by using heavy isotope of nitrogen $ ({{N}^{15}}) $ .