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  4. Other than DNA polymerase, which are the enzymes involved in DNA synthesis?

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Q. Other than DNA polymerase, which are the enzymes involved in DNA synthesis?

AMUAMU 2000

Solution:

Topoisomerase, helicase and RNA primase enzymes are involved in DNA synthesis. They perform following functions :
(i) Topoisomerase reduces the tension, which is produced due to unwinding of DNA strands.
(ii) Helicase or unwindase is the enzyme, which separates $2$ strands of DNA at the site of origin of replication or ori. The separated strands are stabilised by Single Strand Binding Proteins (SSBPs).
(iii) RNA primase or RNA polymerase enzyme: A small strand of RNA $(4-12$ nucleotides) called primer is synthesized at the $3'$ end of each template with the help of this enzyme. RNA primers function as $5'$ ends of new strands. The primer lies at the free end of the template strand and fork end of other template strand.