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Q. Match the items of column-I with those in column-II and choose the correct option.

Column I Column II

A Recombinant DNA technology i Vector

B Cloning vehicles ii Sealing enzyme

C Macromolecular separation iii Electrophoresis

D DNA ligase iv Genetic engineering

Biotechnology : Principles and Processes

A

A - IV; B - I; C - II; D - III

5%

B

A - II; B - I; C - IV; D - III

1%

C

A - IV; B - I; C - III; D - II

94%

D

A - I; B - IV; C - II; D - III

1%

Solution:

A : Recombinant technology is a series of procedures that are used to join together (recombine) DNA segments. A recombinant DNA molecule is constructed from segments of two or more different DNA molecules.
B : Cloning vehicle is the central component of a gene cloning experiment, which transports the gene into the host cell and is responsible for its replication. Two types of DNA molecules that act as vectors are plasmids and bacteriophage (virus chromosomes).
C : Macromolecular separation occurs in electrophoresis. Electrophoresis is a technique for separating the components of a mixture of charged molecules (proteins, DNAs, or RNAs) in an electric field within a gel or other support.
D : DNA ligase is a specific type of enzyme that facilitates the joining of DNA strands together by catalysing the formation of a phosphodiester bond.