Q. In which of the following substrate level phosphorylation does not occur
NTA AbhyasNTA Abhyas 2020
Solution:
Glycolysis / EMP pathway:
(1) Discovery: It was discovered by Embden, Meyerhof and Parnas in 1930. It is the first stage of the breakdown of glucose in the cell.
(2) Definition: Glycolysis (Gr. glykys= sweet, sugar; lysis= breaking) is a stepped process by which one molecule of glucose (6C) breaks into two molecules of pyruvic acid (3C).
(3) Site of occurrence: Glycolysis takes place in the cytosol and does not utilize oxygen. Thus, it is an anaerobic pathway. In fact, it occurs in both aerobic and anaerobic respiration.
Isomerization: Glucose 6-phosphate is changed into its isomer fructose 6-phosphate by rearrangement. The rearrangement is catalyzed by an enzyme, phosphoglucose-isomerase, or phosphohexose isomerase. In sperms, Fructose 6-phosphate may be formed directly from free fructose by its phosphorylation in the presence of an enzyme fructokinase, Mg2+, and ATP. $Glucose6-Phosphate\overset{P h o s p h o h e x o s e i s o m e r a s e}{ \rightarrow }Fructose6-Phosphate$
(1) Discovery: It was discovered by Embden, Meyerhof and Parnas in 1930. It is the first stage of the breakdown of glucose in the cell.
(2) Definition: Glycolysis (Gr. glykys= sweet, sugar; lysis= breaking) is a stepped process by which one molecule of glucose (6C) breaks into two molecules of pyruvic acid (3C).
(3) Site of occurrence: Glycolysis takes place in the cytosol and does not utilize oxygen. Thus, it is an anaerobic pathway. In fact, it occurs in both aerobic and anaerobic respiration.
Isomerization: Glucose 6-phosphate is changed into its isomer fructose 6-phosphate by rearrangement. The rearrangement is catalyzed by an enzyme, phosphoglucose-isomerase, or phosphohexose isomerase. In sperms, Fructose 6-phosphate may be formed directly from free fructose by its phosphorylation in the presence of an enzyme fructokinase, Mg2+, and ATP. $Glucose6-Phosphate\overset{P h o s p h o h e x o s e i s o m e r a s e}{ \rightarrow }Fructose6-Phosphate$