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  4. In the process of transformation, divalent ions are used to make the host competent. The function of such ions is to

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Q. In the process of transformation, divalent ions are used to make the host competent. The function of such ions is to

NTA AbhyasNTA Abhyas 2020Biotechnology : Principles and Processes

Solution:

In order to force bacteria to take up the plasmid, the bacterial cells must first be made ‘competent’ to take up DNA. This is done by treating them with a specific concentration of a divalent cation, such as calcium, which increases the efficiency with which DNA enters the bacterium through pores in its cell wall. Recombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice, followed by placing them briefly at 42°C (heat shock), and then putting them back on ice. This enables the bacteria to take up the recombinant DNA.