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Q.
In RNA interference (RNAi):
NTA AbhyasNTA Abhyas 2020Biotechnology and its Applications
Solution:
This is gene subtraction. This method is used to change the genotype of a plant by either inactivation or removal of the gene. A nematode Meloidogyne incognita infects the roots of tobacco plants causing a great reduction in yield. The strategy adopted to prevent this damage was the RNA interference process (RNAi). RNAi is a naturally occurring mechanism that causes 'silencing' of the genes. This method involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevent translation of mRNA.