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Q.
In plant biotechnology, PEG is used in
Delhi UMET/DPMTDelhi UMET/DPMT 2011Biotechnology : Principles and Processes
Solution:
Somatic hybridization involves the fusion of protoplasts of two different species which resulted in hybrid. Naked protoplasts are obtained by dissolution of their cell walls by the mecerating enzymes such as pectinase and cellulase. Fusion of protoplasts from two different varieties can be enhanced by treating with polyethylene glycol (PEG) in presence of high voltage electric current.