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Q.
In $pBR322$, tetracycline resistance gene $(tet^R)$ has recognition site for which of the following restriction endonuclease?
Biotechnology : Principles and Processes
Solution:
In plasmid vector $pBR322$, two unique restriction sites $Psfl$ and Pvul are located within the $amp^R$ gene and BamHl, Sail, etc., are located within the $tet^R$ gene. The presence of restriction sites within the marker genes $tet^R$ and $amp^R$ permits an easy selection for cells transformed with the recombinant $pBR322$. When restriction enzyme BamHl or Sal I is used, the $DNA$ insert is placed within the gene $tet^R$ making it nonfunctional.