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  4. In Lederberg's replica plating experiment what shall be used to obtain streptomycin resistant strain

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Q. In Lederberg's replica plating experiment what shall be used to obtain streptomycin resistant strain

AIPMTAIPMT 2001Biotechnology : Principles and Processes

Solution:

If streptomycin resistant mutant are to be obtained, material should be allowed to grow on medium lacking streptomycin so that both mutant and wild types may grow. These colonies are imprinted on petriplates to form the master pattern and other plates having streptomycin can then be pressed on velveteen to get an impression. The plate now containing only mutants for streptomycin resistance will grow.