Q.
In $1983$, $Eli\, Lilly$, an American company prepared two $DNA$ sequences corresponding to $A$ and $B$ chains of human insulin and introduced them in plasmids of (a)____ to produce insulin chains. Chains $A$ and $B$ were produced separately, extracted and combined by creating (b)____ to form human insulin. Choose the option which correctly fills in the blank marked as $'a'$ and $'b'$ in the given paragraph.
A
B
A
E.coli
Hydrogen bond
B
E.coli
Phosphodiester bond
C
Ti plasmid
Disulfide bonds
D
E.coli
Disulfide bonds
| A | B | |
|---|---|---|
| A | E.coli | Hydrogen bond |
| B | E.coli | Phosphodiester bond |
| C | Ti plasmid | Disulfide bonds |
| D | E.coli | Disulfide bonds |
Biotechnology and its Applications
Solution: