Question

If pBR322 cloning vector is cut by Pvu I enzyme for the formation of rDNA then which of its marker gene helps in the selection of recombinants?

• A. amp^R gene
• B. tet^R gene
• C. gal gene
• D. Both $\left(\right.A\left.\right)$ and $\left(\right.B\left.\right)$

Answer 1

Answer: (D)

pBR 322 has two selectable markers which are amphicillin and tertacyclin resistant genes. Amphicilin resistant gene has two restriction enzyme recognition sites, Pvu I and Pst I. If the vector is cut by Pvu I enzyme for the formation of rDNA then the amphicillin resistant gene becomes inactive due to insertional inactivation.