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Q.
If a recombinant $DNA$ is inserted within the coding sequence of
$\beta$ -galactosidase enzyme, then
Biotechnology : Principles and Processes
Solution:
• If a recombinant DNA is inserted within the coding sequence of $\beta$-galactosidase enzyme then non recombinant colonies will produce blue colour in presence of chromogenic substrate.
• In this a recombinant DNA is inserted within the coding sequence of an enzyme $\beta$-galactosidase.
• This results into inactivation of the enzyme, which is referred to as insertional inactivation.
• The presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert.
• Presence of insert result into insertional inactivation of the $\beta$ -galactosidase and the colonies do not produce any colour these are identified as recombinant colonies.