Q.
Given figures represent the steps involved in polymerase chain reaction $(PCR)$. Identify the steps $A$, $B$, $C$ and $D$, and select the correct option.
A
B
C
D
(a)
Denaturation
at $94-96^°C$
Extension
through Taq
polymerase at
$72^°C$
Repetition of
denaturation and
annealing
Annealing at
$72^°C$
(b)
Denaturation
at $94-96^°C$
Annealing at
$40-60^°C$
Extension through
Taq polymerase at
$72^°C$
Repetition of
denaturation,
and annealing
(c)
Denaturation
at $40-60^°C$
Annealing at
$72^°C$
Extension through
Taq polymerase at
$94-96^°C$
Repetition of
denaturation
and annealing
(d)
Extension
through Taq
polymerase
at $72°^C$
Denaturation
at $40^°C$
Annealing at $72^°C$
Repetition of
denaturation
and annealing
| A | B | C | D | |
|---|---|---|---|---|
| (a) | Denaturation at $94-96^°C$ | Extension through Taq polymerase at $72^°C$ | Repetition of denaturation and annealing | Annealing at $72^°C$ |
| (b) | Denaturation at $94-96^°C$ | Annealing at $40-60^°C$ | Extension through Taq polymerase at $72^°C$ | Repetition of denaturation, and annealing |
| (c) | Denaturation at $40-60^°C$ | Annealing at $72^°C$ | Extension through Taq polymerase at $94-96^°C$ | Repetition of denaturation and annealing |
| (d) | Extension through Taq polymerase at $72°^C$ | Denaturation at $40^°C$ | Annealing at $72^°C$ | Repetition of denaturation and annealing |
Biotechnology : Principles and Processes
Solution: