Question

Given below is the process to separate the contents of a living tissue by acid treatment and a list of certain observations. Segregate the observations under filtrate(A) and retentate(B) and select the correct combination.

1. Molecular weight ranging from 18-800 daltons approx.
2. Proteins, nucleic acids, polysaccharides and lipids
3. Chemicals more than 800 daltons molecular weight
4. Has monomeres
5. Generally has polymers

• A. (A) : 1, 2, 3 and (B) : 4, 5
• B. (A) : 2, 4 and (B) : 1, 3, 5
• C. (A) : 1, 4 and (B) : 2, 3, 5
• D. (A) : 1, 3, 5 and (B) : 2,

Answer 1

Answer: (C)

The given procedure is about chemical analysis of living matter. Filtrate is the acid soluble pool while the retentate is the acid insoluble pool. Acid soluble pool has biomicromolecules containing low molecular weight molecules having a molecular weight between 18 -800 daltons. They are generally monomers such as amino acids, monosaccharides etc. Acid insoluble pool has biomacromolecules containing high molecular weight molecules having a molecular weight above-800 daltons. They are generally polymers such as carbohydrates, proteins, nucleic acids etc.