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Q.
From the given combinations of steps in PCR, identify the enzyme depended steps
KCETKCET 2020Biotechnology : Principles and Processes
Solution:
Polymerase chain reaction includes three steps - denaturation, annealing and elongation or extension.
Denaturation - the process by which the DNA strands unwind and denature to form two separate strands. This step occurs at a very high temperature.
Annealing - the process by which the DNA primer attaches to the DNA template strand by complementary base pairing.
Elongation - it is the process of addition of nucleotides. A thermostable enzyme called Taq polymerase. This enzyme is extracted from the bacteria called Thermus aquaticus.
