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Q. Flow of electrons in $ETS$ is $NADH+H^{+}\left(NADPH + H^{+}\right) \rightarrow FMN \rightarrow FeS$ protein $ \rightarrow CoQ \rightarrow $ $cytb \rightarrow cytc_{1} \rightarrow cytc \rightarrow cyta \rightarrow cyta_{3} \rightarrow o^{' '}.$ At three steps $ATP$ is formed (oxidative phosphorylation). Where does II $ATP$ is generated?

NTA AbhyasNTA Abhyas 2020

Solution:

The process of forming ATP through the electron transport chain is known as oxidative phosphorylation.
Electrons carried by NADH + H+ and FADH2 are transferred to oxygen via a series of electron carriers, and ATPs are formed. Three ATPs are formed from each NADH + H+, and two ATPs are formed for each FADH2. There are four complexes that are part of the electron transport chain that functions to pass electrons down the chain. A fifth protein complex serves to transport hydrogen ions back into the matrix.
NADH transfers two electrons to Complex-I resulting in four H+ ions being pumped across the inner membrane. NADH is oxidized to NAD+, which is recycled back into the cycle. Electrons are transferred from Complex-I to a carrier molecule ubiquinone (Q), which is reduced to ubiquinol (QH2). Ubiquinol carries the electrons to Complex-III. FADH2 transfers electrons to Complex-II and the electrons are passed along to ubiquinone (Q). Q is reduced to ubiquinol (QH2), which carries the electrons to Complex-III. No H+ ions are transported to the intermembrane space in this process. The passage of electrons to Complex-III drives the transport of four more H+ ions across the inner membrane. QH2 is oxidized and electrons are passed to another electron carrier protein cytochrome C and ATP is generated during the flow of electrons and protons from Cyt b to Cyt c1.