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  4. EcoRI always cuts DNA molecules at a particular point by recognizing a specific restriction sequence, the sticky ends formed after digestion have the sequence

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Q. EcoRI always cuts DNA molecules at a particular point by recognizing a specific restriction sequence, the sticky ends formed after digestion have the sequence

NTA AbhyasNTA Abhyas 2020Biotechnology : Principles and Processes

Solution:

Restriction enzymes, also called restriction nucleases (EcoRI in this question), surround the DNA molecule at the recognition point (sequence GAATTC for EcoRI). EcoRI cuts one strand of the DNA double helix at one point and the second strand at the complementary point (between the G and A base).