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  4. Correct statement about restriction endonuclease. (I) It is the enzyme which cuts textDNA at a particular point. (II) The first restriction endonuclease was textHindII. (III) Overall, text900 restriction enzymes have been isolated from about text200 strains. (IV) Each restriction endonuclease recognizes a specific palindromic sequence.

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Q. Correct statement about restriction endonuclease.
(I) It is the enzyme which cuts $\text{DNA at}$ a particular point.
(II) The first restriction endonuclease was $\text{HindII.}$
(III) Overall, $\text{900}$ restriction enzymes have been isolated from about $\text{200}$ strains.
(IV) Each restriction endonuclease recognizes a specific palindromic sequence.

NTA AbhyasNTA Abhyas 2020Biotechnology : Principles and Processes

Solution:

The first restriction endonuclease HindII whose functioning ws found to be dependent on a specific DNA nucleotide sequence was isolated and characterized 5 years later. It was found that HindII always cuts DNA molecules at a particular point by recognizing a specific sequence of six base pairs. This specific base sequence is known as the recognition sequence for HindII. Besides Hind II, today we know more than 900 restriction enzymes that have been isolated from over 230 strains of bacteria, each of which recognizes different recognition sequences. Each restriction endonuclease recognizes a specific palindromic sequence.