1. Tardigrade
  2. Question
  3. Biology
  4. Commonly used vectors for human genome sequencing are

Question Error Report

Thank you for reporting, we will resolve it shortly

Back to Question

Q. Commonly used vectors for human genome sequencing are

AIPMTAIPMT 2014Biotechnology : Principles and Processes

Solution:

Bacterial artificial chromosome (BAC) vectors are based on natural, extra-chromosomal plasmid of E. coli. BAC vector contains genes for replication and maintenance of the F-factor, a selectable marker and cloning site. These vectors can accommodate upto 300-350 kb of foreign DNA and are also being used in genome sequencing project. Yeast artificial chromosome (YAC) vectors are used to clone DNA fragments of more than 1Mb in size. Therefore, they have been exploited extensively in mapping the large genomes, e.g., in the Human Genome Project. These vectors contain the telomeric sequence, the centromere and the autonomously replicating sequence from yeast chromosomes.