Question

Calculate the limit of resolution of microscope with cone angle of light falling on the objective equal to $60^{\circ}$. (Given $\lambda=5000\,\mathring{A}$, $\mu$ for air $=1)$

• A. $5 \times 10^{-7} m$
• B. $0.5 \mu m$
• C. Both of these
• D. None of these

Answer 1

Answer: (C)

$d =\frac{\lambda}{2 \mu \sin \theta}$
$=\frac{5000 \times 10^{-10}}{2 \times 1 \times \sin 30^{\circ}}$
$\left(2 \theta=60^{\circ}\right) $
$ \left(\theta=30^{\circ}\right)$
$d =5 \times 10^{-7}$
$d =0.5\, \mu m$