1. Tardigrade
  2. Question
  3. Biology
  4. Assertion: Genetic engineering requires both nucleases and ligases. Reason: Ligases produce the nick in the recombinant DNA molecule.

Question Error Report

Thank you for reporting, we will resolve it shortly

Back to Question

Q. Assertion : Genetic engineering requires both nucleases and ligases.
Reason : Ligases produce the nick in the recombinant $DNA$ molecule.

Biotechnology : Principles and Processes

Solution:

Nucleases are the enzymes that remove nucleotides or produce nick in the $DNA$ strand. Exonudeases remove nucleotides from the free ends of $DNA$ while endonucleases produce internal nicks in $DNA$. Now, the desired gene is inserted and then the cut ends are sealed with the help of $DNA$ ligases. Ligases are also called molecular glue as they join together two $DNA$ strands by forming phosphodiester bonds between adjacent nucleotides.