Question

Assertion (A) Foreign DNA and vector DNA cut with the help of ligase.
Reason (R) Ligase acts by forming phosphodiester bonds.

• A. If both A and R are true and R is the correct explanation of A
• B. If both A and R are true, but R is not the correct explanation of A
• C. If A is true, but R is false
• D. If A is false, but R is true

Answer 1

Answer: (D)

Assertion is false, but Reason is true. Assertion can be corrected as
In the formation of rDNA, restriction endonucleases cut both foreign DNA and vector DNA.
Whereas, ligases join two DNA strands by forming phosphodiester bonds between adjacent nucleotides.