Charging or aminoacylation of tRNA is, the process when tRNA specific for the amino acid forms aminoacyl. The amino acid is linked to 3' - OH end of tRNA through its —COOH group. The tRNA complexed with amino acid is sometimes called charged tRNA. This process is essential for protein synthesis i.e., polypeptide formation through formation of peptide bonds between amino acids. The chargedt t RNA carries the amino acid bind to it to the specific codon on mRNA that codes for that particular amino acid. Then bond is formed between this amino acid and the amino acid present already. The free tRNA is released and is now able to carry another amino acid.