Question

According to the chemiosmotic hypothesis of ATP formation in the chloroplast, which one does not contribute to the development of proton gradient across the thylakoid membrane?

• A. Splitting of water
• B. Reduction of $NADP^{+}$ to $NADPH \, + \, H^{+}$
• C. Reduction of $NAD^{+}$ to $NADH \, + \, H^{+}$
• D. Removal of $H^{+}$ from stroma while transporting electron

Answer 1

Answer: (C)

$NAD^{+}$ is not associated with ETS in chloroplast.
According to chemiosmotic hypothesis of ATP formation in chloroplast, reduction of NAD⁺ to NADH + H⁺ does not contribute to develop proton gradient across thylakoid membrane.