Q.
$A$. In elution the separated bands of DNA are cut out from agarose gel and extracted from the gel piece.
$B$. E. coli cloning vector $\rho BR322$ shows several restriction, ri, antibiotic resistance genes and Rop.
$C$. The down stream processing and quality control testing vary from produced to product.
$D$. Competent bacterial cell cannot take up the plasmid.
Biotechnology : Principles and Processes
Solution: