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  4. A DNA fragment was cleaved by using REN and the restriction fragments (a, b, and c) produced as a result of digestion are shown below. Select the option that represents the correct gel electrophoresis separation of these fragments. <img class=img-fluid question-image alt=Question src=https://cdn.tardigrade.in/q/nta/b-3bkj3n2tz5ft.png />

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Q. A DNA fragment was cleaved by using REN and the restriction fragments (a, b, and c) produced as a result of digestion are shown below. Select the option that represents the correct gel electrophoresis separation of these fragments.
Question

NTA AbhyasNTA Abhyas 2020Biotechnology : Principles and Processes

Solution:

In Gel Electrophoresis Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.
hence c been the longest fragment travel short distance.
a and b are short fragment and nearly equal in size ove farther down.