Question

Why are lipids found in the acid insoluble fraction during the analysis of chemical composition of tissues?

• A. It has very high molecular weight
• B. It is polymer
• C. It has low molecular weight
• D. On grinding, the biomembranes are broken into pieces and form insoluble vesicles

Answer 1

Answer: (D)

Upon grinding a tissue, cell membrane and other membranes are broken into pieces and form vesicles, which are not water soluble and get separated along with the acid insoluble pool. Since, lipids are the constituents of cell membrane and are water unsoluble, these also form vesicles and get separated in the acid insoluble pool.