Bordeaux mixture was discovered by Millardet in France in 1882. It is prepared by dissolving 40g of copper sulphate and 40g of calcium hydroxide in 5L of water. It was first used to control downy mildew disease of grape-vine that is caused by a fungus Plasmopara viticola. Black rust of wheat is caused by Puccinia graminis tritici. The use of fungicide Dithane Z−78 and Dithane M−45 control the disease even at lower concentration.
Loose smut of wheat is caused by Ustilago tritici. The use of fungicides (systemic fungicide like D−735 and F−461 ) give quite encouraging result.