Question

Which of the following statements is not correct regarding EcoRI restriction endonuclease enzyme?

• A. It is isolated from Escherichia coli $RY13$
• B. Its recognition sequence is
  $5^{\prime }-GAATTC-3^{\prime }$
  $3^{\prime }-CTTAAG-5^{\prime }$
• C. It produces complementary blunt ends
• D. None of these

Answer 1

Answer: (C)

The restriction endonuclease enzyme EcoRI was isolated from bacterium Escherichia coli $RY13$. It recognises the base sequence $GAATTC$ in $DNA$ duplex and cuts its strands between $G$ and $A$ as shown below :

It results in complementary sticky ends.