Ca2+ is essential of muscle contraction, neutron-muscular functions and nerve impulse transmission. During nerve impulse transmission, as an impulse arrives at a synaptic knob, calcium ions (Ca2+) diffuse into the knobs from surrounding tissue fluid. Ca2+ trigger a process in which numerous synaptic vesicles fuse with the membrane of the knob and the areas of fusion break down; releasing the contents of vesicles into the fluid of synaptic clefts.