Question

Study the given figure carefully and select the incorrect statements regarding this.

(i) It represents a typical agarose gel electrophoresis in which lane $1$ contains undigested $DNA$.
(ii) Smallest $DNA$ bands are formed at $A$ and largest $DNA$ bands are formed at $B$.
(iii) The separated $DNA$ fragments can be visualized after staining in the visible light.
(iv) The separated $DNA$ bands are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.

• A. (i) and (ii)
• B. (ii) and (iii)
• C. (ii) and (iv)
• D. (i) and (iv)

Answer 1

Answer: (B)

The $DNA$ fragments separate according to their size through the agarose gel, with smaller fragments moving farther away as compared to larger ones. The $DNA$ fragments can be visualised by staining them with ethidium bromide followed by exposure to $UV$ radiations.