Question

Select the correct statement about the RNAi method.

• A. RNAi strategy was taken up to prevent the infestation of nematodes in the roots of maize palnts.
• B. This method involves the use of a complementary dsRNA molecule that binds to and prevents translation of the mRNA.
• C. Only RNA genomes of viruses can be used as a source for the complementary RNA.
• D. Mobile genetic elements called transposons can replicate only through a DNA intermediate.

Answer 1

Answer: (B)

Several nematodes parasitise a wide variety of plants and animals including human beings. A nematode Meloidegyne incognitia infects the roots of tobacco plants and causes a great reduction in yield. A novel strategy was adopted to prevent this infestation which was based on the process of RNA interference (RNAi). RNAi takes place in all eukaryotic organisms as a method of cellular defense. This method involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing). The source of this complementary RNA could be from an infection by viruses having RNA genomes or mobile genetic elements (transposons) that replicate via an RNA intermediate.