1. Tardigrade
  2. Question
  3. Biology
  4. Identify the desirable characteristics for a plasmid used in rDNA technology from the following A. Ability to multiply and express outside the host in a bioreactor B. A highly active promoter C. A site at which replication can be initiated D. One or more identifiable marker genes E. One or more unique restriction sites

Q. Identify the desirable characteristics for a plasmid used in rDNA technology from the following
A. Ability to multiply and express outside the host in a bioreactor
B. A highly active promoter
C. A site at which replication can be initiated
D. One or more identifiable marker genes
E. One or more unique restriction sites

 2341  205 KCETKCET 2014Biotechnology : Principles and Processes Report Error

Solution:

Plasmids are extrachromosomal DNA present naturally in a bacterial cell. They are small double-stranded and closed circular DNA. They are used in recombinant DNA technology as cloning and expression vectors. For acting as a vehicle for the desired gene, they should be able to multiply inside the host cell thus need a replication origin site, should have marker genes and unique restriction sites.