At metaphase each chromosome is made up of two sister chromatids held together by the centromere in the centre. During metaphase, kinetochores; disc shaped structures are present at the surface of each centromere which help in the attachment of spindle fibres to the chromosomes. The chromosomes arrange themselves at the equator in one equatorial plane known as metaphase plate. Nucleolus, nuclear envelope, Golgi complex, endoplasmic reticulum disappear during late prophase. Cell plate formation begins during the late anaphase or early telophase of M-phase.