Chemiosmotic hypothesis of ATP forma-tion was propounded by Peter Mitchell in U.K. in 1961. According to it, electron transport, both in respiration and photosynthesis, produces a proton gradient (pH gradient). The gradient develops in the outer chamber or intermembrane space of mitochondria and inside the thylakoid lumen in chloroplasts, resulting in ATP synthesis. According to this hypothesis, ATP synthesis through chemiosmosis requires a membrane, a proton pump, a proton gradient and F0 - F1 particle or ATP-ase.