Assertion: Eli Lily prepared two DNA sequences corresponding to A and B chain of human insulin and introduced them in the plasmid of E.coli to produce polypeptide chains of insulin. Reason: Chains A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin.

Question

Assertion: Eli Lily prepared two DNA sequences corresponding to A and B chain of human insulin and introduced them in the plasmid of E.coli to produce polypeptide chains of insulin. Reason: Chains A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin. (A) If both assertion and reason are true and reason is the correct explanation of assertion (B) If both assertion and reason are true but reason is not the correct explanation of assertion (C) If assertion is true but reason is false (D) If both assertion and reason are false

Tardigrade Admin · Accepted Answer

Insulin is synthesised as a prohormone which contains an extra stretch called C peptide. This C peptide is not present in the mature insulin as it is removed during maturation. The main challenge for insulin production using rDNA technique was getting insulin assembled into a mature form. In 1983, Eli Lily, an American company, first prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. Chain A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin.