According to Llyod (1908), the conversion of starch into sugar in the day time and vice versa at night causes opening and closing of stomata. Sayre (1926), Scarth (1932) suggested that this conversion is controlled only by pH of the guard cells whereas Steward (1964), regards that this is partly enzymatic and partly controlled by pH. The change in the pH of the guard cell's cell sap is due to the presence or absence of CO2 which is dependent on light availability. During day time, in the presence of light, CO2 evolved in respiration is utilized for photosynthesis and hence does not accumulate in the guard cells and pH of the guard cells increases to 7. Hence the enzyme favours the formation of glucose-1-phosphate from starch and inorganic phosphate. Altered sugar concentration increases turgidity of the guard cell due to osmosis and stomata open.